EFFECT OF HYPOXIA ON HIF-1α AND VEGF EXPRESSION AND RADIOSENSITIVITY IN ESOPHAGEAL CANCER CELL LINE Eca109

Objective To investigate the change of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) and radiosensitivity effect by CoCl2 induced hypoxia in esophageal cancer cell line Eca109 in vitro.Methods The hypoxia culture model induced by CoCl2 was established.The gene productions of HIF-1α and VEGF were observed at different hypoxia culture phase.The mRNA expression of HIF-1α and VEGF was shown by reverse transcriptase polymerase chain reaction.The protein of HIF-1α and VEGF was tested using Western Blot analysis.The radiosensitivity was analysized with clonegentic assay after irradiation alone or combined with hypoxia in esophageal caicinoma cell line Eca109 in vitro.Results The mRNA level of HIF-1α was increased at hypoxia culture but was not changed sinificantly with the prolonged hypoxia culture time.However,the protein expression of HIF-1α was increased remarkably with prolonged the hypoxia time;VEGF expression was up regulated under hypoxia both in mRNA and in protein level.The hypoxia decreased the radiosensitivity in espohageal cancer Eca109 cells with clonegenetic assay.Conclusion Expression of HIF-1α protein and VEGF mRNA and protein were up-regulated,the radiosensitivity of Eca109 cell was decreased in hypoxia induced by CoCL2.