Effect of HB-EGF mRNA on Proliferation of Trophoblast in Chinese Herbs for Bromocriptine-Induced Hypoprolactin Rat Abortion Model

Purpose To study expression and correlation of HB-EGF(Heparin-binding epidermiz growth factor),PCNA(proliferating cell nuclear antigen) and influence of proliferation on trophoblast with Chinese Herbs for spontaneous abortion. Methods Bromocriptine-induced hypoprolactin rat abortion model was treated by Chinese Herbs, progesterone and PRL respectively, controls were pure model, normal pregnancy and pseudopregnancy rats, each group contained 12 rats. All rats were killed at pregnant day 12 to observe outcome of pregnancy. Expression and correlation of HB-EGF mRNA and PCNA in trophoblast and endometrium was analyzed by RT-PCR and immunohistochemistry. Microvillus was observed by electron microscope. Results There were no significant differences between expression of HB-EGF and PCNA on trophoblast in the model treated by Chinese Herbs, progesterone, PRL and normal pregnancy(P0.05) . The microvillus was normal in the four groups. Expression of PCNA on trophoblast in these four groups were significantly higher than those of the pure model. Microvillus disappeared in the pure model.There were no expression of HB-EGF on the pure model. Expression of HB-EGF on the oestrum endometrium were significantly higher than those of four groups.There was positive correlation between PCNA and HB-EGF(r=0. 262,P0.01). Conclusions HB-EGF was correlated to proliferation of trophoblast in early pregnancy. Chinese Herbs may enhance expression of HB-EGF on trophoblast through autocrine and paracrine role,and may enhance proliferation, invasion and tolerance anoxia of trophoblast.