Effect of Tianbing Tiaodu capsule on learning and memory and expression of ChAT and AchE in hippocampus of epilepsy rats

OBJECTIVE To observe the effect of Tianbing Tiaodu capsule on learning and memory in epilepsy rats.METHODS Picrotoxin(1.5 mg·kg~(-1)·d~(-1)) was injected intraperitonrally in SD rats for thirty days.After the epilepsy model had been established successfully,the model rats were randomly divided into model group,Tianbing Tiaodu capsule low dosage((1.5 g)·kg~(-1)) group,Tianbing Tiaodu capsules high dosage((4.8 g)·kg~(-1)) group,Piracetam group and Piracetam and Valproate group.All groups were feed everyday.Twenty eight days later,the learning and memory abilities were tested with Morris water maze method.Expression of ChAT and AchE in Hippocampus was observed with immunohistochemical method.RESULTS Compared to the control group,the learning and memory abilities was significantly lower(P(0.01)),immune positive remarks of ChAT in Hippocampus CA_(1) significantly decreased,immune positive remarks of AchE in Hippocampus CA_(2) significantly increased in model group(P(0.05));compared to model group,the learning and memory abilities and immune positive remarks of ChAT and AchE improved in Tianbing Tiaodu group(P(0.05),P(0.01)).CONCLUSION Tianbing Tiaodu capsules could improve the impairment of learning and memory in epilepsy rats.