Expression and purification of recombinant SARS coronavirus spike protein

Aim To clone and express the Spike protein of SARS-CoV.Methods In this work,clone a special gene fragment of S protein of SARS-CoV into vector pET32a.lnduce the expression efficiently by IPTG after the express vector transforms E.coli BL21.Identify the recombinant protein of gain by Western blot.Results The recombinant protein was run through a pillar of nickel to turn pure,to immunize rabbit to get the polyclonal antibody.Conclusion Examined by Elisa,the purified protein can examine the IgG and the IgM in SARS-patient′s serum.It can be further used for the study on Sprotein′s function research and the development of diagnostic method of SARS.