Effect of Tongjingbushen complex prescription on acetylcholine esterase,choline acetyltransferase and acetylcholine in hippocampus of Alzheimer′s disease rat induced by aluminum

Objective To observe the effect of Tongjingbushen complex prescription on acetylcholine esterase(AchE),choline acetyltransferase(ChAT) and acetylcholine(Ach) in hippocampus of Alzheimer′s disease(AD) rat induced by aluminum.Methods 84 3-month-old SD rats were divided into control,model(low,middle and high dose of aluminum),traditional Chinese medicine(low,middle and high dose of traditional Chinese medicine) groups(n=12).Rats of AD were made by adding A1C13·6H2o to normal feed for 3 months.Rats in traditional Chinese medicine groups were given Tongjingbushen traditional Chinese medicine i.g.AchE,ChAT and Ach in hippocampus were detected.Results Compared with control group,AchE activity were higher,ChAT activity were decreased,Ach were increased in middle and high dose model groups(P0.01).Conclusions More uptake of aluminum resulting Ach decrease may be one of nosogenesis of AD,traditional Chinese medicine could increase ChAT activity,inhibit AchE activity,increase Ach content in hippocampus,improve rat central cholinergic system unbalance.