Study on CD95 expression of CD8~+T lymphocytes in patients with chronic hepatitis B

Objective To investigate the expression of CD95 on CD8+T cells by test result and find out the relationship between the expression of CD95 and apotosis of CD8+T cells in chronic hepatitis B patients. Methods Peripheral blood was collected from 37 chronic hepatitis B patients,and density gradient centrifugation was used to isolate PBMCs from peripheral blood. Flow cytometry was employed to test the CD95 expression of CD8+T cells in chronic hepatitis B patients. Results CD95 expression of CD8+T cells in both high viral load group and low viral load group were significantly higher than that in the healthy group( P 0. 05),moreover,the expression of CD95 was negatively related to numbers of CD8+T cell( r =-0. 3486). Conclusions The high expression of CD95 promotes apoptosis of CD8+T cells and weakens CD8+T cell's antiviral effect,which may be associated with the formation of chronic Hepatitis B and the persistent infection of HBV.