Role of renal lymph circulation disorder on renal function and structure in rats

Objective: To investigate the influence of renal lymph circulation disorder on renal function and structure in rats.Methods: Fortyeight male Wister rats were randomly divided into two groups,twenty-four in each: the sham operation group(the control group) and the renal lymph ligation group(the model group).Six rats in each group were sacrificed at 1,2,4 and 8 weeks after operation.Renal tissues were determined by PAS and Masson staining,and renal lesions were determined by semiquantitative scores.The ultra-structure of the renal tissues was determined by an electron microscope.Results: Renal disfunction and tubulointerstitial fibrosis were found in the model group.Tubular damage and tubulointerstitial fibrosis were remarkably aggravated in the model group compared with the control group(P0.01 and P0.05,respectively).Mitochondrion cavitation,mesenterium insertion and foot cell fusion were found in the model group by an electron microscope.Conclusion: These results indicate that a disorder of renal lymph circulation leads to chronic renal failure and structural lesions,especially for tubulointerstitial fibrosis.