in vivo and in vitro Antioxidant Effects of Hesperidin and Chlorogenic Acid

The aim of this study was to investigate the in vitro and in vivo antioxidant effects of hesperidin and chlorogenic acid by pyrogallol autoxidation and fenton reaction and animal studies. Piglets weaned at 14 -28 days were fed basal diet of corn-soybean meal as a control and experiment diets containing 300 mg/kg of hesperidin and 300 mg/kg of chlorogenic acid, respectively for four weeks. The results showed that hesperidin and chlorogenic acid were able to scavenge the oxygen free radicals in vitro in a dose-response manner and chlorogenic acid displayed higher antioxidant activity than hesperidin. Animal studies suggested that chlorogenic acid significantly enhanced the levels of serum CAT, GSH-Px and the CIHR (P 0.05) while hesperidin was able to reduce the contents of MDA (P 0.05).