Inhibition Effect of Ginsenoside Rg_3 on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83

Objective:To explore the effect of Rg3 on inhibiting and inducing apoptosis of human adenoid cystic carcinoma cells.Method: The human adenoid cystic carcinoma cell line SACC-83 was treated with Rg3 of various concentrations.Cell proliferation was measured by MTT assay.Morphological changes of cells were observed by fluorescent microscope.Cell cycle and cell proliferation index were analyzed by flow cytometry(FCM).The expression of hTERT in cells was detected by immunohistochemistry.Result: Rg3 inhibited proliferation of SACC-83 cells in a manner of time-concentration-dependent relationship.When treated with 20 mg·L-1 of Rg3 for 24 h and 48 h,the cells showed apoptotic morphological characteristics including the condensed chromatin,the nuclear fragmentation,the apoptotic body and bright fluorescentgranules.When treated with Rg3 of various concentrations for 72 h,the percentage of cells in G0/G1 phase was increased,whereas the percentage of cells in S phase and G2/M phase was decreased.The cell proliferation index was decreased in a manner of obvious concentration-dependent relationship.The expression of hTERT in cells was inhibited by Rg3.Conclusion: The results suggest that ginsenoside Rg3 exerts an inhibiting effect on proliferation of SACC-83 cells by inducing apoptosis and down regulation of telomerase activity.