Inhibition of siRNA on VEGF expression in SK-BR-3 cells:An experimental study

Objective By using siRNA to downregulate VEGF expression in VEGF positive breast cancer cell line SK-BR-3,and to observe the influence of siRNA of cell proliferation.Method siRNAs targeting VEGF mRNA were obtained by in vitro transcription and transfected into cells with lipofectin;the survival of the transfected cells was detected with MTT assay,the VEGF mRNA level was analyzed with RT-PCR,and the secretion of VEGF protein in the supernatant was detected with ELISA.Results The small interfering RNA (siRNA) targeting human VEGF effectively inhibited the proliferation of breast cancer cell SK-BR-3;the expression of VEGF mRNA was significantly inhibited in SK-BR-3;otherwise,VEGF protein notably decreased,but the effects did not appear in control scramble siRNA.Conclusion siRNA against VEGF could effectively downregulate VEGF expression in SK-BR-3 cell line,inhibit cell growth.siRNAs can effectively inhibit cell growth in human carcinoma cell lines.