Effect on Mitochondrial Membrane Potential and Ca~(2+) Concentration in HepG-2 by CSEO

AIM:To study the mechanism of growth inhibiting and apoptosis inducing effect of total alkaloid in the CSEO(Capparis spinosa L.essential oil,CSEO)on human hepatocarcinoma cell Line HepG-2.Methods:The growth inhibit-ing effect of the CSEO on human hepatocarcinoma cell Line HepG-2 was measured by MTT method.Morphological observa-tion of the HepG-2 cells was completed by fluorescent microscope.The changing of mitochondrion membrane potential in-duced by CSEO was observed by staining with Rhodamine123.Effect of the CSEO on intracellular Ca2+ level of the HepG-2 cells was measured by laser confocal microscope.Results:The CESO has obvious growth inhibiting effect on the HepG-2 and seems to be dose-dependent,and its IC50 is 127.5 μg·mL-1.The characteristic apoptosis morpha of HepG-2 cells was ob-served,and the apoptosis percentage increased to 44.447% in the 300 μg·mL-1 group.In addition,the progress of cells cycle of G1 period was blocked,and the cellular proportion in S and G2 period was decreased in the 75 μg·mL-1 and 150 μg·mL-1 groups by the function of CSEO for 48 h.The mitochondria membrane potential(Δψm)affected by CESO decreased,while the curve moved toward left.In addition,the intracellular Ca2+ level was increased by the function of CESO in the middle and high dose groups.Conclusion:The CESO has obvious growth inhibiting and apoptosis inducing effect on human hepatocar-cinoma cell Line HepG-2 by the mechanism of decreasing the mitochondria membrane potential and increasing the intracellu-lar Ca2+ level.