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2006•Acta Nutrimenta SinicaRequires access

STUDIES ON THE ELIMINATING EFFECT OF RECOMBINANT α-GALACTOSIDASE ON FLATUS INDUCED BY SOYBEAN OLIGOSACCHARIDES IN MICE

Yang-Pei Zhang

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Abstract

Objective: Soy products may cause excessive intestinal gas because of soybean oligosaccharides . The effect of recombinant α-galactosidase on eliminating mouse flatus was observed. Methods:The mouse model of flatulence was set up by ig raffinose and stachyose and the flatulence was investigated by measuring intestinal flatulent volume. Oligosaccharides were examined by TLC test and soybean protein was examined by SDS-PAGE. Results: Raffinose and stachyose can result in mouse flatus and recombinant α-galactosidase can eliminate it without any effect on soybean protein. Conclusion: Recombinant α-galactosidase can eliminate flatus, and be used as food additive.

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What this paper is about

Objective: Soy products may cause excessive intestinal gas because of soybean oligosaccharides . The effect of recombinant α-galactosidase on eliminating mouse flatus was observed. Methods:The mouse model of flatulence was set up by ig raffinose and stachyose and the flatulence was investigated by measuring intestinal flatulent volume. Oligosaccharides were examined by TLC test and soybean protein was examined by SDS-PAGE. Results: Raffinose and stachyose can result in mouse flatus and recombinant α-galactosidase can eliminate it without any effect on soybean protein. Conclusion: Recombinant α-galactosidase can eliminate flatus, and be used as food additive.

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Available abstract

Objective: Soy products may cause excessive intestinal gas because of soybean oligosaccharides . The effect of recombinant α-galactosidase on eliminating mouse flatus was observed. Methods:The mouse model of flatulence was set up by ig raffinose and stachyose and the flatulence was investigated by measuring intestinal flatulent volume. Oligosaccharides were examined by TLC test and soybean protein was examined by SDS-PAGE. Results: Raffinose and stachyose can result in mouse flatus and recombinant α-galactosidase can eliminate it without any effect on soybean protein. Conclusion: Recombinant α-galactosidase can eliminate flatus, and be used as food additive.

Key concepts: Stachyose, Raffinose, Flatulence, Recombinant DNA, Food science, Chemistry, Biochemistry, Biology

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STUDIES ON THE ELIMINATING EFFECT OF RECOMBINANT α-GALACTOSIDASE ON FLATUS INDUCED BY SOYBEAN OLIGOSACCHARIDES IN MICE — Research Paper | ScholarLens