Effects of 5-Aza-Cd R on cell proliferation and DAPK gene expression in human gastric cancer cell line BGC803 cells

Ojective To explore the effects of DNA methylation inhibitor 5-Aza-2'-deoxycytidine(5-Aza-Cd R) on the proliferation, apoptosis and the expression level of tumor suppressor gene DAPK in human gastric cancer cell line BGC803 cells. Methods BGC803 cells were treated with different concentrations of 5-Aza-Cd R and divided into four groups, including control group, 5-Aza-Cd R 1 μmol/L group,5-Aza-Cd R 5 μmol/L group and 5-Aza-Cd R 10 μmol/L group. Cell counting Kit-8 was employed to detect cell proliferation, cell apoptosis was measured by Annexin Ⅴ/PI apoptosis detection kit, RT-PCR was applied to detect the DAPK m RNA expression. Results The proliferation of BGC803 cells was significantly inhibited in a dose-dependent manner compared with control group, after 5-Aza-Cd R treatment, the apoptotic rates of cells increased significantly(P 0.05). The expression levels of DAPK m RNA was gradually increased in a time-dependent manner(P 0.05). Conclusion 5-Aza-Cd R inhibited cells proliferation, promoted cells apoptosis and induced the expression of DAPK gene in BGC803 cells.