Effect of Endogenous Hormones Content Changing on the Chilling Tolerance in Maize Seed Germination
Xuefeng Zhang
Abstract
Xuefeng Zhang
Abstract
Under 10℃ treatment,four maize varieties were used to study the changes of ABA and GA3 endogenous hormones contents on seed germination stage for maize chilling-tolerance.The chilling-resistance cultivars showed more endogenous GA3 contents than chilling-sensitive line,raising and then decreasing at normal and low temperature.The GA3 contents of chilling-tolerance line(Zhengdan958 and Jidan415) increased to the highest level(416.79ng·g-1 and 441.36ng·g-1) after 9d 10℃ treatment.The results indicated that Zhengdan958 and Jidan415 could maintain a higher endogenous ABA(575.2ng·g-1and 596.52ng·g-1)and lower GA3/ABA under low temperature during seed germinating.Tiedan18 and Liaodan632 had more dramatically changes than Zhengdan958 and Jidan415.This also indicated that these indexes may affect chilling-tolerance among different cultivars.Effects of exogenous hormones on the seed germination vigor and seedlings growth were tested to improve chilling-tolerance at low temperature.The best resist-chilling concentration is GA35mg·L-1,ABA10mg·L-1.
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Under 10℃ treatment,four maize varieties were used to study the changes of ABA and GA3 endogenous hormones contents on seed germination stage for maize chilling-tolerance.The chilling-resistance cultivars showed more endogenous GA3 contents than chilling-sensitive line,raising and then decreasing at normal and low temperature.The GA3 contents of chilling-tolerance line(Zhengdan958 and Jidan415) increased to the highest level(416.79ng·g-1 and 441.36ng·g-1) after 9d 10℃ treatment.The results indicated that Zhengdan958 and Jidan415 could maintain a higher endogenous ABA(575.2ng·g-1and 596.52ng·g-1)and lower GA3/ABA under low temperature during seed germinating.Tiedan18 and Liaodan632 had more dramatically changes than Zhengdan958 and Jidan415.This also indicated that these indexes may affect chilling-tolerance among different cultivars.Effects of exogenous hormones on the seed germination vigor and seedlings growth were tested to improve chilling-tolerance at low temperature.The best resist-chilling concentration is GA35mg·L-1,ABA10mg·L-1.
Key concepts: Germination, Endogeny, Hormone, Cultivar, Gibberellin, Biology, Agronomy, Abscisic acid