Establishment of Taxol-resistance ovarian carcinoma cell line OV1228/Taxol

OBJECTIVE:To establish a taxol-resistance cell line of human ovarian carcinoma(OV1228/taxol),and investigate its biological features.METHODS:With the sensitive OV1228 human ovarian cancer cell line as the parental cells,taxol-resistant cell line of human ovarian cancer(OV1228/taxol) was established by continuous stepwise selection in the increasing concentration of taxol.Cell morphology,growth curve and population doubling time,drug sensitivity,cell cycle,cell uptake of Rh-123,as well as mRNA and protein levels of MDR1 and PKC-α were investigated to determine the biological features of OV1228/Taxol cell line.RESULTS: The resistance index(RI) of OV1228/Taxol cells was up to 39.44.They showed an obvious cross-resistance to DDP and 5-FU.Compared with OV1228 cells,OV1228/Taxol resistant cells were significantly heteromorphosis,and the population doubling time was significantly longer(P=0.033 5).The results of uptake of Rh-123 further proved that OV1228/Taxol cells had a significant resistance to Rh-123(P=0.005 4).PT-PCR and Western bolt studies showed that the levels of PKC-α and MDR1 mRNA and protein expression in the OV1228/Taxol cells were higher than those in the sensitive cells.CONCLUSION: OV1228/Taxol cell line shows typical multidrug resistance phenotypes and might serve as an ideal model for exploring the new route of reversing multidrug resistance.