Antiglioma effect of dendritic cells derived from human cord blood in vitro

Objective To investigate the antiglioma e ffect of the CD34+ dendritic cells derived from human cord blood in vitro. Methods CD34+ mononuclear cells were isolated from neonatal cor d blood by ficoll centrifugation and magnetic se-paration technique, then cultu red with cytokines (SCF,FL-3,GM-CSF,IL-4,TNF-α). After two weeks of culture , the cells were checked by FACS. The allo-MLR and CTL were detected by MTT me thod.Results (1) CD34+ mononuclear cells from cord blood exh ibited distinctive morphological features of dendritic cell after culture. (2) F low cytometry demonstrated CD40, CD80, CD86 expressed on the mature DCs. (3)Allo -mixed lymphocyte reaction showed that DCs from cord blood stimulated by tumor antigens (SHG-44) induced stronger CTL reaction. Conclusion C D34 dendritic cells from cord blood are capable of inducing antiglioma respons e in vitro.