Effects of AG490 on the expression of CD40 and CD45 and neurological functional status after brain injury in rats

Objective To investigate the effects of AG490 on the expression of CD40 and CD45 in cerebral tissue and neurological functional status after traumatic brain injury in rats. Methods A total of180 healthy male SD rats were randomly allocated into a sham operation,a traumatic brain injury,and an AG490 intervention group( n = 60 in each group). The rats in each group were redivided into 6,12,24and 72 h subgroups according to the time of brain injury. A brain injury model was induced by using a hydraulic shock method. The CD40 and CD45 expression in brain tissue was detected by flow cytometry. The rat neurological deficit score was used to judge the neurological status. Results ①The neurological deficit scores of all time points in the traumatic brain injury group were higher than those in the sham operation group( P 0. 05 or P 0. 01),it reach the peak at 6 h,and then gradually downward. The neurological deficit scores in the AG490 intervention group were lower than those in the traumatic brain injury group.There was significant difference between the time points of 24 h and 72 h( P 0. 05 or P 0. 01). ②The CD40 expression level in brain tissue in the traumatic brain injury group increased gradually,it reached the peak at 24 h,and then it began to decrease; the CD45 expression level increase continuously,it reached the peak at 72 h. Compared with the sham operation group,there were significant differences at each time point between the CD40 and CD45 expression( P 0. 01); the CD40 and CD45 expression levels at each time point in the AG490 intervention group were lower than those in the traumatic brain injury group. There were significant differences( P 0. 01). Conclusion After traumatic brain injury,AG490 may promote the recovery of neurological function by reducing CD40 and CD45 expression in brain tissue.