Effects of insulin-like growth factor binding protein-6 on proliferation and migration of RPE cell

Objective To study the effects of insulin-like growth factor binding protein-6(IGFBP-6) on proliferation and migration of retinal pigment epithelial(RPE) cells.Methods ARPE-19 cells were cultured with IGF-Ⅱ(50 mg·L-1),PDGF(20 mg·L-1),VEGF(40 mg·L-1) and TGF-β(4 mg·L-1) for 6 hours to induce the cell proliferation,then IGFBP-6(1 mg·L-1,10 mg·L-1,100 mg·L-1,500 mg·L-1,1000 mg·L-1)were added.OD value was tested by MTS method after 24 hours and 48 hours to detect the effect of IGFBP-6 on cell proliferation.The cell scratch test was divided into IGF-Ⅱ(50 mg·L-1) group,IGFBP-6+IGF-Ⅱgroup(IGFBP-6 500 mg·L-1,IGF-Ⅱ 50 mg·L-1) and serum free medium group,the wound healing rate was observed at 24 hours and 48 hours,respectively to detect the effect of IGFBP-6 on cell migration.Results The MTS method outcomes indicated that IGFBP-6(100 mg·L-1,500 mg·L-1,1000 mg·L-1)could inhibit the proliferation of ARPE-19 induced by IGF-Ⅱ(P0.05),but not by PDGF,VEGF,TGF-β(all P0.05).Would healing rate in IGF-Ⅱgroup,IGFBP-6+IGF-Ⅱgroup,serum free medium group was(43.91±3.85)%,(29.76±2.49)%,(26.12±2.33)% at 24 hours and(66.09±1.67)%,(59.88±3.43)%,(57.05±2.49)% at 48 hours,there were statistical differences(all P0.05).Conclusion IGFBP-6 can inhibit the proliferation and migration of ARPE-19 cells induced by IGF-Ⅱ.