Analysis on sputum bacterial cultures and drug resistance in 146 aged inpatients

Objective To investigate the distribution and antibiotic resistance status of clinical isolates in our hospital during 2009 to guide rational drug use in clinic.Methods Kirby-Bauer method was used to conduct the susceptibility testing and ESBLs detection.The results were analyzed by WHONET 5.4 software according to CLSI 2006.Results Of the 134 clinical isolates,79.1%(106/134)were Gram-negative bacilli and 20.9%(28/134)were Gram-positive cocci.E.coli was the most frequently isolated pathogen in Gram-negative bacilli.Staphylococcus haemolyticus was the most common pathogen in Gram-positive cocci.ESBLs were produced in 37.5% of E.coli and 21.4% of K.pneumoniae isolates respectively.About 50% of S.aureus isolates were MRSA,91.3% of coagulase negative Staphylococcus isolates were methicillin-resistant.A strain of high level aminoglycoside resistance E.faecalis was identified.Conclusion Antimicrobial resistance in clinical bacterial isolates is a very serious problem in our hospital.Antimicrobial agents should be used rationally based on the results of susceptibility testing.