Bimolecular Fluorescence Complementation Technique

Biomolecular fluorescence complementation (BiFC) is a recently developed new technique to detect the protein-protein interactions in vitro and in vivo. The technique is based on the principle that two nonfluorescent fragments of a fluorescent protein dissected at appropriate site are brought together and reconstructed to fluorescence, depending on the association or interaction between the proteins fused to each fragment. BiFC method not only enables directly visualization and localization of protein interactions but also is simple and noninvasive. Moreover, multiple color BiFC systems or combing BiFC and fluorescence resonance energy transfer (FRET) technology may be exploited to detect the multiple protein-protein interactions.