The Study of Inhibitory Effect of VPA Combined with Paclitaxel on Cervical Cancer Hela Cells

Objective To explore the inhibitory effect of histone deacetylase inhibitor VPA combined with paclitaxel on human cervical cancer Hela cells.Methods Hela cells were treated with VPA and paclitaxel alone and in combination.The cell condition was observed by microscope.Cell proliferation was studied by MTT assay.The apoptosis and cell cycle were determined by flow cytometry.Results The Hela cells were significantly inhibited by VPA in a time and dose-dependent manner,and VPA induced enhanced apoptosis and G2/M phase arrest.When combined with paclitaxel,VPA induced more severe apoptosis and arrested much more cells at G2/M phase;meanwhile the cells of S phase were decreased.Conclusion VPA can significantly inhibit the proliferation of Hela cells and induce apoptosis,enhancing paclitaxel's killing effect on Hela cells.