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Expression of PSP94-TNF αD11a Fusion Gene in NIH3T3 Cells and Bioassays of the Expressed Products

Qing Liu

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Abstract

To identify the expression and the biological activity of human PSP94 TNF αD11a,NIH3T3 cells were transfected by pcDNA\|PSP94 TNF αD11a.Total RNA from the transfected cells was analyzed by RT\|PCR.The products showed the expected length and could also hybridized with PSP94 probe.The results of Western blot and ELISA indicated that the transfected NIH 3T3 cells could express PSP94 TNF α D11a in the culture medium.Activity assay of the culture medium from the transfected NIH 3T3 showed not only suppressive effects of PSP94 on human prostate coarcinoma cell PC\|3 growth,but also cytotoxicity of TNF α on L929 cells.These results suggested that PSP94 TNF αD11a could be correctly expressed in NIH 3T3 cells,and the expressed products had the expected double effects on tumor cells.

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What this paper is about

To identify the expression and the biological activity of human PSP94 TNF αD11a,NIH3T3 cells were transfected by pcDNA\|PSP94 TNF αD11a.Total RNA from the transfected cells was analyzed by RT\|PCR.The products showed the expected length and could also hybridized with PSP94 probe.The results of Western blot and ELISA indicated that the transfected NIH 3T3 cells could express PSP94 TNF α D11a in the culture medium.Activity assay of the culture medium from the transfected NIH 3T3 showed not only suppressive effects of PSP94 on human prostate coarcinoma cell PC\|3 growth,but also cytotoxicity of TNF α on L929 cells.These results suggested that PSP94 TNF αD11a could be correctly expressed in NIH 3T3 cells,and the expressed products had the expected double effects on tumor cells.

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Available abstract

To identify the expression and the biological activity of human PSP94 TNF αD11a,NIH3T3 cells were transfected by pcDNA\|PSP94 TNF αD11a.Total RNA from the transfected cells was analyzed by RT\|PCR.The products showed the expected length and could also hybridized with PSP94 probe.The results of Western blot and ELISA indicated that the transfected NIH 3T3 cells could express PSP94 TNF α D11a in the culture medium.Activity assay of the culture medium from the transfected NIH 3T3 showed not only suppressive effects of PSP94 on human prostate coarcinoma cell PC\|3 growth,but also cytotoxicity of TNF α on L929 cells.These results suggested that PSP94 TNF αD11a could be correctly expressed in NIH 3T3 cells,and the expressed products had the expected double effects on tumor cells.

Key concepts: Transfection, Cell culture, 3T3 cells, Tumor necrosis factor alpha, Molecular biology, Biology, Northern blot, Gene expression

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Expression of PSP94-TNF αD11a Fusion Gene in NIH3T3 Cells and Bioassays of the Expressed Products — Research Paper | ScholarLens