Inducing chitinase and #beta#-1,3-glucanase activities of melon by spraying inducer
LI Xue-wen, Ling Xu, Hui Zhang, Qinghua Cai
Abstract
LI Xue-wen, Ling Xu, Hui Zhang, Qinghua Cai
Abstract
In the greenhouse the first leaf of melon was separately sprayed with BTH and Harpin. With the chitinase and β-1,3-glucanase activities, first, second and fourth leaves were detected after two days of spraying inducer. The results showed that the level of chitinase and β-1,3-glucanase activity were all induced. The chitinase reached its peak after seven days of sprarying, then descended. The β-1,3-glucanase reached its peak after five days of handling, then declined. But each of enzymes activity is different due to different inducer and concentration. This experiment showed that the chitinase and β-1,3-glucanase activity in the tissue of melon which did not spray inducer were induced, the domino offect can be transferred by transfer tissue .
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In the greenhouse the first leaf of melon was separately sprayed with BTH and Harpin. With the chitinase and β-1,3-glucanase activities, first, second and fourth leaves were detected after two days of spraying inducer. The results showed that the level of chitinase and β-1,3-glucanase activity were all induced. The chitinase reached its peak after seven days of sprarying, then descended. The β-1,3-glucanase reached its peak after five days of handling, then declined. But each of enzymes activity is different due to different inducer and concentration. This experiment showed that the chitinase and β-1,3-glucanase activity in the tissue of melon which did not spray inducer were induced, the domino offect can be transferred by transfer tissue .
Key concepts: Chitinase, Glucanase, Inducer, Melon, Chemistry, Enzyme, Enzyme assay, Biology