2006•Chinese Journal of NeonatologyRequires access

The expression of cyclooxygenase-2 in neonatal rat with hypoxic-ischemic brain damage

Haiyan Li

Abstract

Objective To study the role of cyclooxygenase-2 (COX-2) in neonatal rat with hypoxic-ischemic brain damage (HIBD). Methods With the neonatal rat model of HIBD, hypoxia time was 2 hours, the expression of COX-2 mRNA was analyzed by RT-PCR, and COX-2 protein expression was studied by immunohistochemistry. The expression of COX-2 mRNA and COX-2 protein were studied at 6, 24, 48 hours and 5 days after hypoxia. Results COX-2mRNA expression increased after HIBD, (0.461±0.052),(0.887±0.0816),(0.660±0.119),(0.474±0.104)respectively. The difference was significant compared with control group(0.321±0.175), P0.01. The peak appeared at 24 hours after HIBD, and it was higher than other time points. Similar time course of COX-2 protein expression was seen, (42.50±75.88),(837.50±88.84),(510.00±49.67),(242.50±47.89). Compared with control group, (21.75±12.84), P0.01. Conclusions COX-2 may play a role in neonatal HIBD development.

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Key concepts: Medicine, Brain damage, Hypoxia (environmental), Cyclooxygenase, Immunohistochemistry, Messenger RNA, Andrology, Protein expression