Comparison and analysis of SCoT and ISSR markers for genetic diversity of longan

The diversity and genetic relationship among and within thirty-six varieties of longan and one Dimocarpus confinis from China,Vietnam and Thailand were analyzed using SCoT(Start Codon Targeted) and ISSR(Inter Simple Sequence Repeat) markers.The results showed 127 strips were amplified by 12 SCoT primers while 117 strips by 15 ISSR primers.The average strips amplified by SCoT and ISSR primers were 10.58 and 7.8,respectively.The genetic relationships were analyzed using unweighted pair-group method of arithmetic average cluster analysis(UPGMA)and the genetic Jaccard similarity coefficients were calculated.The cluster analysis results showed that the thirty-seven materials could be clustered into 6 groups at the similarity coefficient level of 0.672 and 0.685.Comparison and mixture analysis of the two molecular markers demonstrated that both SCoT and ISSR were efficient approaches for genetic diversity analysis of longan germplasm.These results would provide theoretical basis for longan germplasm storage and utilization in future.