siRNA inhibits MDM2 expression and proliferation of hepatoma carcinoma cell

Objective Using siRNA to downregulate MDM2 expression in hepatocellular carcinoma cell line hepG2,to observe the influence of siRNA of cell proliferation and apoptosis.Methods MDM2-siRNAs against the MDM2 gene were designed and transfected into hepG2 cells respectively,MDM2 and P53 gene and protein expression was measured using RT-PCR and western blot,Cell proliferation was measured with MTT assay,the cell cycle were analysed using flow cytometer.Results MDM2 mRNA and protein expression were inhibited and P53 mRNA and protein expression were enhanced after transfecting with two siRNA MDM2 respectively.As a consequence,cell growth inhibition was observed,cell cycle change was observed after transfecting with two siRNA MDM2 too.Conclusion siRNA against MDM2 could effectively downregulate MDM2 and upregulate P53 expression in hepG2 cell line,inhibit cell proliferation and change cell cycle.