ScholarLens Back to search
2007•Heilongjiang xumu shouyiRequires access

Construction of H5 subtype Avian Influenza HA recombinant baculovirus expressive vector

Hualan Chen

Open publisher page 0 citations

Abstract

The HA gene of H5 subtype AIV was amplify by RT-PCR from the DK/Zhejiang/11/00(H5N1) and stepped into pFastBac plasmid,the recombinant plasmid was transforming DH10Bac competent cells to get the positive recombinant bacmid.Recombinant baculovirus were harvest by transfecting bacmid to sf 9 cells.PCR confirmed that HA gene had inserted into the baculovirus genome.Indirect immunofluorescence assay(IFA),SDS-PAGE analysis and Western-blot test showed that H5HA gene has been expressed in sf 9 cells infected with the recombinant baculovirus,and which proved the expressed protein have good biological activity and special immunogenicity.

About this research paper

What this paper is about

The HA gene of H5 subtype AIV was amplify by RT-PCR from the DK/Zhejiang/11/00(H5N1) and stepped into pFastBac plasmid,the recombinant plasmid was transforming DH10Bac competent cells to get the positive recombinant bacmid.Recombinant baculovirus were harvest by transfecting bacmid to sf 9 cells.PCR confirmed that HA gene had inserted into the baculovirus genome.Indirect immunofluorescence assay(IFA),SDS-PAGE analysis and Western-blot test showed that H5HA gene has been expressed in sf 9 cells infected with the recombinant baculovirus,and which proved the expressed protein have good biological activity and special immunogenicity.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The HA gene of H5 subtype AIV was amplify by RT-PCR from the DK/Zhejiang/11/00(H5N1) and stepped into pFastBac plasmid,the recombinant plasmid was transforming DH10Bac competent cells to get the positive recombinant bacmid.Recombinant baculovirus were harvest by transfecting bacmid to sf 9 cells.PCR confirmed that HA gene had inserted into the baculovirus genome.Indirect immunofluorescence assay(IFA),SDS-PAGE analysis and Western-blot test showed that H5HA gene has been expressed in sf 9 cells infected with the recombinant baculovirus,and which proved the expressed protein have good biological activity and special immunogenicity.

Key concepts: Recombinant DNA, Plasmid, Virology, Immunogenicity, Biology, Molecular biology, Gene, Western blot

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction of H5 subtype Avian Influenza HA recombinant baculovirus expressive vector — Research Paper | ScholarLens