Detection of aminoglycosides resistance condition and the relative genes of pseudomonas aeruginosa

Objective To investigate aminoglycosides resistance condition and its mechanism.Methods Antibiotics sensitivity tests were taken by microdilute method,and five genes of aminoglycoside-modifying enzymes were detected and sequenced by PCR.Results The resistant rates of the 264 strains pseudomonas aeruginosa to gentamyci,tobramycin and amikacin were 8.6%,7.5%,2.9% respectively.In 6 of 20 strains of clinically infective pseudomonas aeruginosa,aminoglycoside-modifying enzymes genes that were respectively aac(3)-II(2/20),aac(6′)-Ⅰ(1/20),ant(3)-Ⅰ(1/20) and ant(2)-Ⅱ(2/20) were detected.Conclusion The rate of sensitivity to aminoglycosides in pseudomonas aeruginosa isolated from our hospital is high.These antibiotics remain priority choice in clinical treatment in pseudomonas aeruginosa infections.Amimoglycoside-modifying enzymes genes of pseudomonas aeruginosa might be one of mechanisms of resistance to aminoglycosides.