Establishment of Duplex PCR for Detecting Aeromonas veronii

The duplex PCR was developed with genome DNA of Aeromonas veronii ATCC35624 as template and the primers designed according to 16SrRNA and nuclease gene(exu gene).The sensitivity and specificity were verified and meanwhile the simulation polluted samples were detected by this method.The results showed that the DNA fragments of approximate 880bp and 320bp were obtained.Both 16SrRNA gene and exu gene shared 99% homology with these genes from Aeromonas veronii ATCC35624.This method had high sensitivity with the detected DNA concentration approximate 1.58×10-4 ng/μL and high specificity with only the standard strains been detected.The artifical simulation polluted experiments showed that the detected rate of this method with 90% was higher than it of bacterial cultivation with 73.3%.The developed duplex PCR can overcome the shortage of traditional bacterial cultivation and provide the new method to detect Aeromonas veroni.