Effects of ulinastatin on IL-6,IL-8 and TNF-α mRNA expression during one lung ventilation

AIM:To investigate the effects of ulinastatin on expression of IL-6,IL-8 and TNF-α mRNA during one lung ventilation.METHODS: Thirty patients with lung cancer ⅢA after chemotherapy ASA Ⅱ-Ⅲ,selected to pulmonary surgery,were included in this study and were randomly divided into two groups: control group(group C,n=15) and ulinastatin group(group U,n=15).Patients in group U received ulinastatin(10 000 U·kg-1,iv) after induction of anesthesia.Patients in group C: received equal volume of normal saline,instead of ulinastatin.The lung tissue far from the tumor was taken and washed two times by PBS.Total RNA was extracted from the lung tissue using a standard Trizol protocol and expression of IL-6,IL-8 and TNF-α mRNA were detected by RT-PCR.RESULTS: The expression of IL-6,IL-8 and TNF-α mRNA in two groups were low after induction of anesthesia.They increased significantly 90 min after one lung ventilation in both two groups.Compared with control group,the expression of IL-6,IL-8 and TNF-α mRNA in the ulinastatin group decreased significantly 90 min after one lung ventilation.CONCLUSION: The expression of IL-6,IL-8 and TNF-α mRNA during one lung ventilation increase significantly,and ulinastatin depresse the expression of these cytokines at the transcriptional levels and reduces lung injury.