Effect of arginine enriched TPN on gut barrier of rats with acute pancreatitis

Objectives:To investigate the effect of arginine enriched TPN on gut barrier of rats with acute pancreatitis. Methods:64 SD rats were divided into four groups at random:1.Control group(sham operated, n =16);2.AP group( n =16);3.AP+TPN group(TPNs group, n =16);4.AP+TPN+Arginine group (TPNa group, n =16).Every 8 rats in each group were sacrificed on day 1 and day 5 after inducing acute pancreatitis respectively.The level of nitric oxide(NO),malondialdehyde,protein in intestinal mucosa and bacterial translocation rates were measured. Results:Compared with control group on day 1 and day 5 after induction of acute pancreatitis.The level of malondialdehyde in intestinal mucosa and bacterial translocation rates of mesenteric lymphaden of AP group increased significantly( P 0.01, P 0.05);The level of protein in intestinal mucosa decreased and bacterial translocation rates in portal vein increased significantly on day 5( P 0.01).Compared with TPNs group,the level of NO in intestinal mucosa increased and bacterial translocation rates of mesenteric lymphaden of TPNa group decreased significantly on day 1( P 0.05).On day 5,the level of NO and protein in intestinal mucosa increased significantly( P 0.05, P 0.01),and malondialdehyde decreased significantly( P 0.01). Conclusion:Acute pancreatitis may cause the damage of gut barrier;Arginine enriched TPN has protective effect on gut barrier of rats with acute pancreatitis.