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Study on Cryopreservation of Mouse 2-Cell Embryos by Straw or OPS Vitrification

Tan Hong-ming

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Abstract

The results of cryopreservation of mouse 2-cell embryos by straw and OPS vitrification method in this study showed that when the 2-cell embryos were equilibrated in EFS40 at 20℃ for 1 min (one-step straw method) before cryopreservation, the blastocysts rate was only 35.0%. There was a significant difference (P0.01) from the control ((65.0%)). When 2-cell embryos were pretreated in 10% EG+10% D for 5 min then treated in EFS for 30 s (two-step), the blastocysts rates were 47.7%～48.8%. At 25℃, the blastocysts rate for two-step method was 52.2%. There was no significant difference (P0.05) compared with the control. When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.2%, the highest rate in all groups. After the blastocysts cultured from thawed embryos from the best straw group and OPS group were transferred, all groups had obtained offspring.

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What this paper is about

The results of cryopreservation of mouse 2-cell embryos by straw and OPS vitrification method in this study showed that when the 2-cell embryos were equilibrated in EFS40 at 20℃ for 1 min (one-step straw method) before cryopreservation, the blastocysts rate was only 35.0%. There was a significant difference (P0.01) from the control ((65.0%)). When 2-cell embryos were pretreated in 10% EG+10% D for 5 min then treated in EFS for 30 s (two-step), the blastocysts rates were 47.7%～48.8%. At 25℃, the blastocysts rate for two-step method was 52.2%. There was no significant difference (P0.05) compared with the control. When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.2%, the highest rate in all groups. After the blastocysts cultured from thawed embryos from the best straw group and OPS group were transferred, all groups had obtained offspring.

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Available abstract

The results of cryopreservation of mouse 2-cell embryos by straw and OPS vitrification method in this study showed that when the 2-cell embryos were equilibrated in EFS40 at 20℃ for 1 min (one-step straw method) before cryopreservation, the blastocysts rate was only 35.0%. There was a significant difference (P0.01) from the control ((65.0%)). When 2-cell embryos were pretreated in 10% EG+10% D for 5 min then treated in EFS for 30 s (two-step), the blastocysts rates were 47.7%～48.8%. At 25℃, the blastocysts rate for two-step method was 52.2%. There was no significant difference (P0.05) compared with the control. When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.2%, the highest rate in all groups. After the blastocysts cultured from thawed embryos from the best straw group and OPS group were transferred, all groups had obtained offspring.

Key concepts: Vitrification, Straw, Cryopreservation, Blastocyst, Andrology, Embryo, Significant difference, Embryo cryopreservation

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