The expression and significance of epididymis protein P34h in obstructive azoospermia patients

Objective: To explore the expression and significance of P34h in the patients with obstructive azoospermia.Methods:Reverse transcription-polymerse chain reaction(RT-PCR) and Western blot assay were used to examine different mRNA and protein expressions of P34h in epididymis tissues in 30 cases of obstructive azoospermia due to different obstruction sites which located at the vas deferens or ejaculatory duct.Results: The mRNA expression level of P34h in epididymis tissues was different.P34h mRNA expression of the group with obstructive site at vas deferens was lower than that with obstructive site at ejaculatory duct(mean value of P34h/?茁-actin:0.418 ± 0.045 vs.0.719 ± 0.069,P 0.05).P34h protein in the group with obstruction at vas deferens was significantly lower than that with obstruction at ejaculatory duct(protein:0.095 ± 0.014 vs.0.276 ± 0.054,P 0.05).Conclusion: The mRNA and protein expressions of P34h were significantly different in epididymis tissues due to various obstructive factors.It may play an important role in sperm mature obstacles in the patient with obstructive azoospermia.