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The application of suppression subtractive hybridization in gene cloning

Fenglan Huang

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Abstract

Suppression subtractive hybridization is a method that combine suppression PCR with subtractive hybridization technology.It can be used to clone the difference analysis gene of unbeknown list.It possess some traits of quick speed,lower false positive rate,aim gene high enrichment and experiment result uncomplicated.This paper outline its principle,the process of manipulation,advantage,disadvantage and application in gene cloning,and analyses its application in the future.

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What this paper is about

Suppression subtractive hybridization is a method that combine suppression PCR with subtractive hybridization technology.It can be used to clone the difference analysis gene of unbeknown list.It possess some traits of quick speed,lower false positive rate,aim gene high enrichment and experiment result uncomplicated.This paper outline its principle,the process of manipulation,advantage,disadvantage and application in gene cloning,and analyses its application in the future.

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Available abstract

Suppression subtractive hybridization is a method that combine suppression PCR with subtractive hybridization technology.It can be used to clone the difference analysis gene of unbeknown list.It possess some traits of quick speed,lower false positive rate,aim gene high enrichment and experiment result uncomplicated.This paper outline its principle,the process of manipulation,advantage,disadvantage and application in gene cloning,and analyses its application in the future.

Key concepts: Suppression subtractive hybridization, Subtractive color, Cloning (programming), Biology, clone (Java method), Genetics, Gene, Computational biology

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