2004•Unpublished venueRequires access
Expression of Smad 3, 7 in Rats Renal Tissue after Unilateral Ureteral Obstruction and Effects of Shentong Capsule on Them
Jiacai Hu
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Abstract
Objective To investigate the expression of TGF-β1 and Smad 3, 7 in rats renal tissue after unilateral ureteral obstruction(UUO) and the effect of Shentong capsule on their expressions. Methods Sixteen rats underwent UUO and were devided into operation group and Shentong treatment group, additional 8 rats were shame operation group. The pathological change of renal tissues were examined by light microscopy 8 days after operation. The extent of renal fibrosis was determined by measuring the area of renal interstitial fibrosis. Immunhistochemistry was applied to detect the expression of TGF-β1 and Smad 3,7. Results In comparison with shame operation group, the expression of TGF-β1 and Smad 3 in operation group significantly increased, and the expression of Smad 7 significantly decreased(P0 01). In comparison with operation group, in Shentong treatment group the expression of TGF-β1 and Smad 3 significantly decreased, and the expression of Smad 7 significantly increased (P0 05). Conclusion Shentong capsule may decelarate renal interstitial fibrosis by inhibiting the over-expression of Smad 3 and promoting the expression of Smad 7 to down-regulate the expression of TGF-β1.