The effect of manganese on P450scc in rat Leydig cells

Objective To investigate the effect of manganese on P450scc in rat Leydig cells.Methods After establishment of primary culture method of rat Leydig cells,different concentrations of MnCl2(0,10,30,100 μmol /L) were added into cultured cell medium,24 hours later,the contents of Mn in cell culture supernatant and in Leydig cells were detected by atomic absorption spectrometry(AAS),The contents of testosterone,P450scc in cell culture supernatant and P450scc in Leydig cells were measured by ELISA,The expression of P450scc mRNA was quantified by RT-PCR.Results Compared with the control group,The contents of Mn in Leydig cells and testosterone in cell culture supernatant increased gradually,at the doses of 30,100 μmol /L,the differences were significant(P 0.05).The content of P450scc increased gradually,at the doses of 30,100 μmol /L,the differences were significant in Leydig cells(P 0.05);and at the doses of 10,30,100 μmol /L,the differences were significant in cell culture supernatant(P 0.05).The expression of P450scc mRNA increased and the difference was significant at the dose of 100 μmol /L(P 0.05).Conclusions MnCl2 could upregulate the expression of P450Scc gene,increase the content of P450Scc,and promote the production of testosterone of Leydig cells in vitro.