2002•Letters in BiotechnologyRequires access
Construction and immunological evaluation of DNA vaccine of UreB and HspA of Helicobacter pylori
Xiuli Liu
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Abstract
To testify the effect of DNA vaccine of Helicobacter pylori,the Urease B and Heat-shock protein A were chose as candidate antigens.Amplified by PCR,the UreB and HspA gene were cloned into the plasmid pCDNA3.1(-)His-Myc.Immunized through muscle injection,the Bal B/c mice produced the antisera against UreB or HspA.