THE EXPERIMENTAL STUDY ON THERAPY FOR CERVIX CANCER-BEARING MICE BY INJECTING IL-2 IN THE SPACE PERILYMPHAMATICALLY

Objective:To verify the feasibility and effect of biotherapy institued via the space perilymphamatically on gynecological cancer.Method:A cervix cancer-bearing BALB/C mice were constracted by transplanted U14 cell line.The cancer-bearing mice were divided into four groups.Treated by IL-2,5-Fu,IL-2+5Fu or NS separaetly.After 10 days of treatment,10 mices of each group were killed and their spleen cells were separated to assay the activation of NK,CTL.Weighting the the tumor tissues to compute the percentage of inhibition.The other mice were feeded as previously to observe their survival days.Result: The activations of NK are:NS:(13.23±4.82)%;IL-2:(35.68±5.65)%;5-Fu:(15.67±4.66)%;IL-2+5Fu:(33.33±5.01)%;The activations of CTL are:NS:(17.63±3.75)%;IL-2:(27.34±6.15)%;5-Fu:(19.62±5.17)%;IL-2+5-Fu:(24.01±6.23)%.The percentage of tumor inhibition are:IL-2:36.87%;5-Fu:42.91%;IL-3+5-Fu:48.85%.The average survival days are: NS:12.7±3.12;IL-2:17.5±4.00;5-Fu:19.7±4.72;IL-5+5-Fu:20.6±5.21.Conclusion:It can promote the activity of NK and CTL,inhibite the growth of tumor cells and prolong the survival time of cervix cancer bearing mice by injecting IL-2 cytokine in the space perilymphamatically.