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A Study on the Rooting of Virus-free Pyrus.spp Shoots in Vessel

Qing Jin, Cai Yongping, Lin Yi, Shaojun Ma

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Abstract

The experiment on rooting capacity of virus-free Pyrus.spp tissue cultured shoots,was carried out by applying the orthogonal design or by three different rooting modes in vessel.The results showed: phloroglucinol was the very important factor affecting the rooting in vessel.The ASH medium with 80 mg/L PG,1 mg/L IBA,0.5 mg/L NAA was the most suitable.With two-step rooting mode,the rooting percentage,number,length and speed were significantly higher than those of direct rooting method,and the rooting percentage was up to 87.5 %.

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What this paper is about

The experiment on rooting capacity of virus-free Pyrus.spp tissue cultured shoots,was carried out by applying the orthogonal design or by three different rooting modes in vessel.The results showed: phloroglucinol was the very important factor affecting the rooting in vessel.The ASH medium with 80 mg/L PG,1 mg/L IBA,0.5 mg/L NAA was the most suitable.With two-step rooting mode,the rooting percentage,number,length and speed were significantly higher than those of direct rooting method,and the rooting percentage was up to 87.5 %.

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Available abstract

The experiment on rooting capacity of virus-free Pyrus.spp tissue cultured shoots,was carried out by applying the orthogonal design or by three different rooting modes in vessel.The results showed: phloroglucinol was the very important factor affecting the rooting in vessel.The ASH medium with 80 mg/L PG,1 mg/L IBA,0.5 mg/L NAA was the most suitable.With two-step rooting mode,the rooting percentage,number,length and speed were significantly higher than those of direct rooting method,and the rooting percentage was up to 87.5 %.

Key concepts: Shoot, Phloroglucinol, Biology, Horticulture, Botany

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