Biological effects of liposome-mediated c-myc antisense oligodeoxynucleotide on human hepatoma cells

Objective To study the biological effects of lipofect AMINE reagent (LR) mediated c myc antisense oligodeoxynucleotide(ASODN) on human hepatoma cell SMMC 7721.Methods We transfected 7721 cell with the compound of 1 mg/L LR and 2 μmol/L c myc ASODN to investigate the effets of c myc ASODN on it.Results By MTT method assay,the growth inhibition rate (55%) of LR ASODN group on hepatoma cell is significantly increased comparing with the growth inhibition rate (15%) of no LR group ( P 0.01) .Immunohistochemical and in situ PCR assay showed that c myc expression was decreased in transfected cells.Through electron microscopic observation,it was found that a part of human hepatoma cells changed into apoptosis cells.Conclusion LR mediated c myc ASODN provided a significant growth inhibition on human hepatoma cell,and probably induced the cell apoptosis.