The Study of Detection of Human Papillomavirus Type 16 DNA in Cervical Tissues by Semiquantitative PCR

Objective To study the relationship between the quantities of HPV16 E 6gene in cervical tissues and the course of cervical disease.Methods The HPV16 E 6gene was detected by semi-quantitative PCR in 44 HPV16 positive samples including 20 cases of chronic cervicitis,6 cases of cervical dysplasia and 18 cases of cervical cancer.Results The quantities of HPV16 E 6gene were significantly different between cervical dysplasia,cervical cancer and chronic cervicitis(P0.01).There was no significant difference between cervical dysplasia and cervical cancer(P0.05).Conclusion The quantities of HPV16 E 6gene increase as lesions progress toward cervical cancer.Quantification of HPV16 DNA may be useful to identify women who are at high risk of developing cervical cancer.