Separation of activated Compounds from Synechococcus sp. and the Bioactivities

Marine microalga Synechococcus is one of Cyanobacteria . In order to investigate the bioactivity components of Synechococcus , three organic solvent petroleum ether , ethyl acetate and normal butanol were used to extract the ethanol extractum of Synechococcus , respectively , and three kinds of organic phase crude extracts were obtained. Then the antibio- sis and antioxidant activities of crude extracts were detected. The chemical components of crude extracts were analyzed by GC-MS chromatogram , and 20 components of petroleum ether phase and normal butanol phase were determined , re- spectively. The activity tracking results showed that the best antibacterial and antioxidant effects of crude extracts were observed in petroleum ether phase , followed by ethyl acetate and normal butanol phase. The aqueous extracts had not bio- activities. Subsequently , Silica gel column chromatography , gel Sephadex LH-20 chromatography and lamina chromatogra- phy were used to separate and purify the compound from petroleum ether phase , and a monomeric compound was ob- tained , the structure of compound was elucidated as β -sitosterol by the means of