2006Journal of Hebei UniversityRequires access

Mutagenesis Breeding of the Feed Enzyme-complex Producing Strain

Shiying Wang

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Abstract

One feed complex-enzyme producing mutant strain of Aspergillus niger JW001 was obtained by UV mutagenesis,the strain can produce high activity of multi-enzyme systems.The cellulase enzyme activity was 18 379 u/g,122% higher than original strain and the acidic proteinase enzyme activity was 6 472 u/g,92%higher than original strain.The solid plate fermentation process of this strain was optimized and animal feeding experiments were carried out,the results showed that the feed enzyme-complex produced by strain JW001 could bring series beneficial effects on different animals.

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One feed complex-enzyme producing mutant strain of Aspergillus niger JW001 was obtained by UV mutagenesis,the strain can produce high activity of multi-enzyme systems.The cellulase enzyme activity was 18 379 u/g,122% higher than original strain and the acidic proteinase enzyme activity was 6 472 u/g,92%higher than original strain.The solid plate fermentation process of this strain was optimized and animal feeding experiments were carried out,the results showed that the feed enzyme-complex produced by strain JW001 could bring series beneficial effects on different animals.

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Available abstract

One feed complex-enzyme producing mutant strain of Aspergillus niger JW001 was obtained by UV mutagenesis,the strain can produce high activity of multi-enzyme systems.The cellulase enzyme activity was 18 379 u/g,122% higher than original strain and the acidic proteinase enzyme activity was 6 472 u/g,92%higher than original strain.The solid plate fermentation process of this strain was optimized and animal feeding experiments were carried out,the results showed that the feed enzyme-complex produced by strain JW001 could bring series beneficial effects on different animals.

Key concepts: Strain (injury), Aspergillus niger, Enzyme, Cellulase, Enzyme assay, Mutagenesis, Fermentation, Mutant

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