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Cloning,high prokaryotic expression and purification of human growth hormone

Yong Ge

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Abstract

By the method of RT PCR,the cDNA of human growth hormone(HGH)was amplified from the CHO cells transfected with the full length DNA of HGH gene.The cDNA was cloned into the vector pET 11b followed by transforming the E.coli BL21(DE3).The recombinant HGH was expressed by the induction of IPTG at a ratio of 20% of total proteins.After chromatograph,the purity of rHGH is higher than 95%.

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What this paper is about

By the method of RT PCR,the cDNA of human growth hormone(HGH)was amplified from the CHO cells transfected with the full length DNA of HGH gene.The cDNA was cloned into the vector pET 11b followed by transforming the E.coli BL21(DE3).The recombinant HGH was expressed by the induction of IPTG at a ratio of 20% of total proteins.After chromatograph,the purity of rHGH is higher than 95%.

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Available abstract

By the method of RT PCR,the cDNA of human growth hormone(HGH)was amplified from the CHO cells transfected with the full length DNA of HGH gene.The cDNA was cloned into the vector pET 11b followed by transforming the E.coli BL21(DE3).The recombinant HGH was expressed by the induction of IPTG at a ratio of 20% of total proteins.After chromatograph,the purity of rHGH is higher than 95%.

Key concepts: Complementary DNA, Recombinant DNA, Cloning (programming), Molecular biology, lac operon, Transfection, Growth hormone, Expression vector

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