Experimental investigates on changes of expression of Aquaporin2(AQP2 ) in renal of rats with obstructive jaundice

Objective: To investigate the change of expression of AQP2 in renal of obstructive jaundice rats,and the relationship between AQP2 and the changing of Cr and BUN. Methods: Legated the common bile duct of rats to form the obstructive jaundice group. Scarified the rats on the 3th,5th,7th,10 th,14th day,took blood samples and the kidney of the rats. Test direct bilirubin BUN and Cr in serum,the renal histopathological changes were observed by optical microscopy. The expression of AQP2 in renal of rats was tested by using radio immunological method. Results: Light microscopic examination of kidney showed that swelling epithelium arranged irregularly in 3rd day,bleb in the 5th day. The expression of AQP2 in renal of rats with obstructive jaundice were greatly less than sham operation. Mesenchyma inflammatory cells infiltrate in 7th day. Local epithelial necrosis and lots of inflammatory cells infiltrate in the mesenchyma in 10 th day and 14 th day. The expression of AQP2 in renal collective tubule was decreased on the 5th day compared with sham operation,and the decrease was more as time gone by. The renal function injury can be confirmed though the renal collective tubule change. Cr and BUN in serum began to increase on the 10th day and 14th day. Conclusion: Decrease of AQP2 is earlier than the increase of Cr and BUN,and it can be the early sign of renal function injury.