Effects of normal human bone marrow fibroblastoid stromal cell line on the proliferation of HL-60/VCR multidrue resistant cell line

Objective To investegate the effects of normal human bone marrow fibroblastoid stromal cell line(HFCL) on the proliferation and differentiation of acute myeloid leukemia multidrug resistant (MDR) cell line HL-60/VCR. Methods Drug sensitive test was shown by MTT assay. Establishing coculture system of leukemia MDR cell line HL-60/VCR and HFCL cell, growth curves were detected by cell counting. Cell differentiation was determined by morphologic observation, ability of NBT cells and flow cytometric detection of the expression of CD11b,CD14,CD13 and CD33. Flow cytometry was also performed to test the change of cell cycle. Western blot was used to study the expression of proliferating cell nuclear antigen (PCNA) and P glycoprotein(P-gp). Results HL-60/VCR cells were multidrug resistant. Compared with HL-60/VCR cells without HFCL cells, the proliferation of HL-60/VCR cells in direct contact with HFCL cells and with HFCL cells separated by transwell was inhibited. And NBT positive cells increased slightly. The percentage of G1 phase cells of HL-60/VCR cells with HFCL cells was higher than that without HFCL cells, and the percentage of S phase cells was lower. Meanwhile the expression of CD11b and CD14 increased, however, the expression of CD13 and CD33 didn't change. The expression of PCNA in HL-60/VCR cells with HFCL cells was lower than HL-60/VCR cells without HFCL cells. The expression of P-gp of HL60/VCR cells didn't change in the condition of coculture. Conclusion The normal bone marrow fibroblastoid stromal cells HFCL could inhibit the proliferation and progress of cell cycle of acute myeloid leukemia MDR cells HL-60/VCR, and also could induce the differentiation of part of HL-60/VCR cell into monocyte.