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2009•Anhui nongye kexueRequires access

Immunohistochemical Localization of Growth Differentiation Factor 9 in Rabbit Ovaries

Lin Li

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Abstract

[Objective] To investigate the regulatory mechanism of growth differentiation factor 9(GDF9)during ovarian folliculogenesis.[Method] The localization of GDF9 protein in rabbit ovaries was examined through immunohistochemical method.[Result] The GDF9 protein was not expressed in primordial follicles;it was found in oocytes of primary,secondary and antral follicles,granulosa cells of antral follicles,zona pollucida,follicular flu and corpora lutea.[Conclusion] The expression of GDF9 in rabbit ovaries varies dynamically with time and space to follow folliculogenesis.Besides,GDF9 is not only the cytokine of oocytes but also that of granulosa cells,which plays a significant role in the process of ovarian folliculogenesis and participates in the regulatory activity of corpora lutea.

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What this paper is about

[Objective] To investigate the regulatory mechanism of growth differentiation factor 9(GDF9)during ovarian folliculogenesis.[Method] The localization of GDF9 protein in rabbit ovaries was examined through immunohistochemical method.[Result] The GDF9 protein was not expressed in primordial follicles;it was found in oocytes of primary,secondary and antral follicles,granulosa cells of antral follicles,zona pollucida,follicular flu and corpora lutea.[Conclusion] The expression of GDF9 in rabbit ovaries varies dynamically with time and space to follow folliculogenesis.Besides,GDF9 is not only the cytokine of oocytes but also that of granulosa cells,which plays a significant role in the process of ovarian folliculogenesis and participates in the regulatory activity of corpora lutea.

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Available abstract

[Objective] To investigate the regulatory mechanism of growth differentiation factor 9(GDF9)during ovarian folliculogenesis.[Method] The localization of GDF9 protein in rabbit ovaries was examined through immunohistochemical method.[Result] The GDF9 protein was not expressed in primordial follicles;it was found in oocytes of primary,secondary and antral follicles,granulosa cells of antral follicles,zona pollucida,follicular flu and corpora lutea.[Conclusion] The expression of GDF9 in rabbit ovaries varies dynamically with time and space to follow folliculogenesis.Besides,GDF9 is not only the cytokine of oocytes but also that of granulosa cells,which plays a significant role in the process of ovarian folliculogenesis and participates in the regulatory activity of corpora lutea.

Key concepts: Folliculogenesis, Growth differentiation factor-9, Antral follicle, Follicular phase, Ovary, Biology, Immunohistochemistry, Internal medicine

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