Display of globular domain of HN protein of Newcastle disease virus on bacteriophage T4 capsid surface

HN gene with length of 1 713 bp of Newcastle disease virus was amplified by RT-PCR. The PCR product was purified and cloned into pGEM-T Easy vector and the recombinant plasmid was (sequenced). According to the HN gene sequencing result, another pair of primers containing EcoRⅠrestriction site was designed to amplify the HN fragment coding the globular domain, i.e. the main functional (region) of HN protein. Then the HN fragment was inserted into the 3′ terminal of SOC gene of pSOC plasmid. The recombinant plasmid pSOC-HN was conducted into E.coli BL-21(DE3) to induce HN gene expression with 1 mmol/L IPTG. A SOC-HN fusion protein band with molecular weight of 67 ku was dectected on the SDS-PAGE gel and nitrocellulose membrane,and the expression products can specifically reacted with the antisera against Newcastle disease virus.