2008•Zhongguo meijie shengwuxue ji kongzhi zazhiRequires access
Detection and sequence analysis of Borrelia burgdorferi sensu lato DNA in rat-shape animals from Zhejiang province
Wang Zhi-gan
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Abstract
Objective To investigate the natural infection situation of Borrelia burgdorferi sensu lato in rat-shape animals from Zhejiang province.Methods The segment between 5S rRNA and 23S rRNA of B.burgdorferi sensu lato in the rat-shape animals was amplified with the method of nest-PCR,and the positive PCR products were sequenced and analyzed.Results 128 rat-shape animals were captured in Jindong district of Jinhua city.12 positive samples were detected in Rattus,Apodemus and Shrew,and the positive rate were 9.68%,14.28% and 25.00%,respectively.The sequence analysis results showed the amplified segments from two positive samples were homologous to that of B.valaisiana and their gene serial numbers were AB091447BvalaiOS115 and DQ188933BvalaiQX13P25.The comparability reached 100% between them.Conclusion It suggested that there was B.burgdorferi sensu lato in Zhejiang province,and the rice field mouse,Apodemus and shrew could be the hosts of B.valaisiana.But it still needs be further studied.