Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain
Lihui Liu
Abstract
Lihui Liu
Abstract
Taking diseased plants of Betonica of ficinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.
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Taking diseased plants of Betonica of ficinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.
Key concepts: Sclerotium, Pathogen, Biology, Trichoderma harzianum, Trichoderma, Officinalis, Antagonism, Pathogenicity