2013Northern HorticultureRequires access

Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain

Lihui Liu

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Abstract

Taking diseased plants of Betonica of ficinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.

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What this paper is about

Taking diseased plants of Betonica of ficinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.

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Available abstract

Taking diseased plants of Betonica of ficinalis as experimental materials,the pathogen was identified by pathogenicity test;the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis;the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc;among the five tested trichoderma strains,Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis,its antagonistic index and control efficiency were Ⅱ level and 86.27%,respectively.

Key concepts: Sclerotium, Pathogen, Biology, Trichoderma harzianum, Trichoderma, Officinalis, Antagonism, Pathogenicity

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